Proteomics for biomarker discovery

MING, Z ed
cover image
Added Author
TIMOTHY, V ed
Publication
New Jersey, Humana Press 2013
ISBN
9781627033602 / 9781627033596
Physical Description
xi 320p
Series
Methods in Molecular Biology Vol 1002
URL
Language
eng
 
 
 
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$a With the advent of proteomics came the development of technologies, primarily mass spectrometry, which allowed high-throughput identification of proteins in complex mixtures.  While the mass spectrometer resides at the heart of proteomics, its ability to characterize biological samples is only as good as the sample preparation and data analysis tools used in any study.  In Proteomics for Biomarker Discovery, expert researchers in the field detail many of the methods which are now commonly used to study proteomics. These include methods and techniques include both label-free approaches and those that utilize stable isotopes incorporated both during cell growth or added via a chemical reaction once the proteome is extracted from the cell. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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Subject
E-BOOKS
METHODS AND PROTOCOLS
PROTEOMICS
Summary
With the advent of proteomics came the development of technologies, primarily mass spectrometry, which allowed high-throughput identification of proteins in complex mixtures.  While the mass spectrometer resides at the heart of proteomics, its ability to characterize biological samples is only as good as the sample preparation and data analysis tools used in any study.  In Proteomics for Biomarker Discovery, expert researchers in the field detail many of the methods which are now commonly used to study proteomics. These include methods and techniques include both label-free approaches and those that utilize stable isotopes incorporated both during cell growth or added via a chemical reaction once the proteome is extracted from the cell. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Corresp. Author
1. Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, Inc. Frederick National Laboratory for Cancer Research, Frederick MD USA
2. Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, Inc. Frederick National Laboratory for Cancer Research, Frederick MD USA
Notes
Springer Protocols