Methods in enzymology Vol 584: Enzymology at the membrane interface: Intramembrane proteases

GELB, M H ed
cover image
Publication
USA Elsevier Inc / Academic Press 2017
ISBN
9780128122136
Physical Description
xix 474p
Series
Methods in Enzymology
URL
Language
eng
 
 
 
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$a Volume 584 of Methods in Enzymology is focused on proteolytic enzymes that access their transmembrane peptide substrates in the membrane phase (interfacial proteases).  Biochemical characterization of bacterial and eukaryotic trans-membrane proteases is covered in Chapters 1, 2, 4, 5, 6, 7, 10, and 15.  Kinetic studies including substrate specificity and inhibition are included in Chapters 2, 4, 5, 6, 9, 11, 12, and 15. Studies that probe the structure of trans-membrane proteases are given in Chapters 3, 6, 8, and 13.  Production of recombinant trans-membrane proteases is the specific focus of Chapters 5 and 10.
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Subject
E-BOOKS
ENZYMOLOGY
METHODS AND PROTOCOLS
PROTEOLYTIC ENZYMES
INTERFACIAL ENZYMES
TRANS-MEMBRANE PROTEASES
Summary
Volume 584 of Methods in Enzymology is focused on proteolytic enzymes that access their transmembrane peptide substrates in the membrane phase (interfacial proteases). Biochemical characterization of bacterial and eukaryotic trans-membrane proteases is covered in Chapters 1, 2, 4, 5, 6, 7, 10, and 15. Kinetic studies including substrate specificity and inhibition are included in Chapters 2, 4, 5, 6, 9, 11, 12, and 15. Studies that probe the structure of trans-membrane proteases are given in Chapters 3, 6, 8, and 13. Production of recombinant trans-membrane proteases is the specific focus of Chapters 5 and 10.