Ion Channel Localization: Methods and Protocols

LOPATIN, A N ed.
cover image
Added Author
NICHOLS, C G ed.
Publication
Totowa, NJ : Humana Press, 2001.
ISBN
9781592591183
Printed edition: 9780896038332
Physical Description
XVII, 502 p. online resource.
Series
Methods in Pharmacology and Toxicology
URL
Language
eng
 
 
 
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$a Pharmacological Labeling of Ion Channels and Receptors -- Fluorescent Calcium Antagonists Tools for Imaging of L-Type Calcium Channels in Living Cells -- Targeting Cerebral Muscarinic Acetylcholine Receptors with Radioligands for Diagnostic Nuclear Medicine Studies -- GABAA Receptors -- Localization of ATP P2X Receptors -- Small Conductance Calcium-Activated Potassium Channels in Rat Brain -- Mapping N-Type Calcium Channel Distributions with H-Conotoxins -- Fluorescent Imaging of Nicotinic Receptors During Neuromuscular Junction Development -- High Affinity Scorpion Toxins for Studying Potassium and Sodium Channels -- Applications of Green Fluorescent Protein (GFP) Technology -- Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells -- Confocal Imaging of GFP-Labeled Ion Channels -- Localization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus Oocytes -- Applications of Green Fluorescent Protein (GFP) Technology -- Tagging Ion Channels with the Green Fluorescent Protein (GFP) as a Method for Studying Ion Channel Function in Transgenic Mouse Models -- Mutant GFP- Based FRET Analysis of K+ Channel Organization -- Delivering Ion Channels to Mammalian Cells by Membrane Fusion -- Functional Assays in Ion Channel Localization -- Detection of Neurons Expressing Calcium- Permeable AMPA Receptors Using Kainate-Induced Cobalt Uptake -- Crystallization Technique for Localizing Ion Channels in Living Cells -- Imaging of Localized Neuronal Calcium Influx -- Using Caged Compounds to Map Functional Neurotransmitter Receptors -- Atomic Force Microscopy -- Atomic Force Microscopy of Reconstituted Ion Channels -- Imaging the Spatial Organization of Calcium Channels in Nerve Terminals Using Atomic Force Microscopy -- Nanoarchitecture of Plasma Membrane Visualized with Atomic Force Microscopy -- Dynamic AFM of Patch Clamped Membranes -- Localizing Ion Channels with Scanning Probe Microscopes: A Perspective.
520
$a Although there are many useful books on virtually every aspect of ion channel structure and function, work on channel localization-determining the specific sites of ion channels and their receptors on cells-has long been neglected. In Ion Channel Localization: Methods and Protocols, active researchers at the cutting-edge of their fields describe how to use current and developing technologies to determine the exact cell sites of such channels and receptors. With emphasis on techniques employing the unique properties of these proteins, the core topics of the book include all the major pharmacological tools for ion channel determination. Also covered are fluorescent and radioligand assays, exciting new visualization methods employing green fluorescent protein (GFP), and novel assays based on the functional properties of ion channels and receptors, as well as rapidly developing atomic force microscopy methodologies. Comprehensive and rich in practical detail, Ion Channel Localization: Methods and Protocols brings together in a single volume detailed reviews of all the major techniques-both currently in use and in development-for localizing ion channels and receptors in cells, tissues, and organisms.
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Subject
MEDICINE
PHARMACOLOGY
BIOMEDICINE
METHODS AND PROTOCOLS
E-BOOKS
Summary
Although there are many useful books on virtually every aspect of ion channel structure and function, work on channel localization-determining the specific sites of ion channels and their receptors on cells-has long been neglected. In Ion Channel Localization: Methods and Protocols, active researchers at the cutting-edge of their fields describe how to use current and developing technologies to determine the exact cell sites of such channels and receptors. With emphasis on techniques employing the unique properties of these proteins, the core topics of the book include all the major pharmacological tools for ion channel determination. Also covered are fluorescent and radioligand assays, exciting new visualization methods employing green fluorescent protein (GFP), and novel assays based on the functional properties of ion channels and receptors, as well as rapidly developing atomic force microscopy methodologies. Comprehensive and rich in practical detail, Ion Channel Localization: Methods and Protocols brings together in a single volume detailed reviews of all the major techniques-both currently in use and in development-for localizing ion channels and receptors in cells, tissues, and organisms.
Table of Contents
Pharmacological Labeling of Ion Channels and Receptors -- Fluorescent Calcium Antagonists Tools for Imaging of L-Type Calcium Channels in Living Cells -- Targeting Cerebral Muscarinic Acetylcholine Receptors with Radioligands for Diagnostic Nuclear Medicine Studies -- GABAA Receptors -- Localization of ATP P2X Receptors -- Small Conductance Calcium-Activated Potassium Channels in Rat Brain -- Mapping N-Type Calcium Channel Distributions with H-Conotoxins -- Fluorescent Imaging of Nicotinic Receptors During Neuromuscular Junction Development -- High Affinity Scorpion Toxins for Studying Potassium and Sodium Channels -- Applications of Green Fluorescent Protein (GFP) Technology -- Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells -- Confocal Imaging of GFP-Labeled Ion Channels -- Localization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus Oocytes -- Applications of Green Fluorescent Protein (GFP) Technology -- Tagging Ion Channels with the Green Fluorescent Protein (GFP) as a Method for Studying Ion Channel Function in Transgenic Mouse Models -- Mutant GFP- Based FRET Analysis of K+ Channel Organization -- Delivering Ion Channels to Mammalian Cells by Membrane Fusion -- Functional Assays in Ion Channel Localization -- Detection of Neurons Expressing Calcium- Permeable AMPA Receptors Using Kainate-Induced Cobalt Uptake -- Crystallization Technique for Localizing Ion Channels in Living Cells -- Imaging of Localized Neuronal Calcium Influx -- Using Caged Compounds to Map Functional Neurotransmitter Receptors -- Atomic Force Microscopy -- Atomic Force Microscopy of Reconstituted Ion Channels -- Imaging the Spatial Organization of Calcium Channels in Nerve Terminals Using Atomic Force Microscopy -- Nanoarchitecture of Plasma Membrane Visualized with Atomic Force Microscopy -- Dynamic AFM of Patch Clamped Membranes -- Localizing Ion Channels with Scanning Probe Microscopes: A Perspective.
Notes
Springer Protocols
Methods in Pharmacology and Toxicology, 1557-2153