Macromolecular Crystallography Protocols: Volume 1, Preparation and Crystallization of Macromolecules

WALKER, J M ed
cover image
Added Author
DOUBLIE, S ed.
Publication
Totowa, NJ : Humana Press, 2007.
ISBN
9781588292926 / eISBN:9781597452090
Printed edition: 9781588292926
Physical Description
XIV, 280 p. 49 illus. online resource.
Series
Methods in Molecular Biology Vol 363
URL
Language
eng
 
 
 
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$a A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag -- Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project -- Baculoviral Expression of an Integral Membrane Protein for Structural Studies -- Protein Engineering -- Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems -- How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals -- Screening and Optimization Methods for Nonautomated Crystallization Laboratories -- Improving Marginal Crystals -- Optimization Techniques for Automation and High Throughput -- Three-Dimensional Crystallization of Membrane Proteins -- Crystallization of Protein-DNA Complexes -- Preparation and Crystallization of RNA -- Crystallization of RNA-Protein Complexes.
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$a Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.
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$a CELL BIOLOGY
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Subject
LIFE SCIENCES
BIOCHEMISTRY
CELL BIOLOGY
METHODS AND PROTOCOLS
E-BOOKS
Summary
Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.
Table of Contents
A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag -- Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project -- Baculoviral Expression of an Integral Membrane Protein for Structural Studies -- Protein Engineering -- Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems -- How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals -- Screening and Optimization Methods for Nonautomated Crystallization Laboratories -- Improving Marginal Crystals -- Optimization Techniques for Automation and High Throughput -- Three-Dimensional Crystallization of Membrane Proteins -- Crystallization of Protein-DNA Complexes -- Preparation and Crystallization of RNA -- Crystallization of RNA-Protein Complexes.
Notes
Springer Protocols
Methods in Molecular Biology, 1064-3745 ; 363