Methods in enzymology vol 589:enzymes as sensors

THOMPSON, R B ed
cover image
Added Author
FIERKE, C A ed
Publication
USA Elsevier Inc / Academic Press 2017
ISBN
9780128054062
Physical Description
xviii 511p
Series
Methods in Enzymology
URL
Language
eng
 
 
 
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$a The Editors are proud to present this volume of Methods in Enzymology, focused largely on Enzymes as Sensors. They are fortunate to have gathered a constellation of star investigators to describe their current work in this field. The editors (and many of the authors) have felt for some years that for sensing and many other tasks, biomolecules, such as enzymes, have outstanding attributes. Indeed, biomolecules now represent the method of choice for recognition of many analytes and transducing their presence or level as a signal one can interpret. In this case, necessity has been the mother of invention because answering the essentially analytical questions in biology of how much of an analyte is present, how fast is that amount changing, and how is it distributed in the matrix (often of a living cell), and collecting this information in real time on a submicron scale is a challenge beyond most traditional analytical methods. The proportion of the chapters herein describing methods focused on answering precisely such difficult questions is an index of the inherent power of enzymes and other biological molecules to address such questions.
541
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Subject
ENZYMOLOGY
ENZYMES
BIOMOLECULES
METHODS AND PROTOCOLS
E-BOOKS
Summary
The Editors are proud to present this volume of Methods in Enzymology, focused largely on Enzymes as Sensors. They are fortunate to have gathered a constellation of star investigators to describe their current work in this field. The editors (and many of the authors) have felt for some years that for sensing and many other tasks, biomolecules, such as enzymes, have outstanding attributes. Indeed, biomolecules now represent the method of choice for recognition of many analytes and transducing their presence or level as a signal one can interpret. In this case, necessity has been the mother of invention because answering the essentially analytical questions in biology of how much of an analyte is present, how fast is that amount changing, and how is it distributed in the matrix (often of a living cell), and collecting this information in real time on a submicron scale is a challenge beyond most traditional analytical methods. The proportion of the chapters herein describing methods focused on answering precisely such difficult questions is an index of the inherent power of enzymes and other biological molecules to address such questions.